Electron Ionization–Induced Release of Coded Isotopic Reporter Ions in an m/z Zone of Minimal Interference for Quantifiable, Multiplexed GC-MS Analyses

摘要: We describe an isotope coding strategy that enables simultaneous GC-MS analysis of multiple samples for substrate identification and quantification. The method relies on direct measurement isotopic ethyl carbenium ions serving as mass spectral tags in a zone minimal interference (ZMI) at m/z 31–37. Sample aldehyde ketone mixtures were reacted with 2-aminooxyethyl propionates to illustrate the method, which determined relative abundance mixed compounds average 95% accuracy. ZMI reporter ion detection also chemoselective profiling absolute quantification, demonstrated using biologically derived sample.