作者: Emma Westas , Martin Gillstedt , Jessica Lönn-Stensrud , Ellen Bruzell , Martin Andersson
DOI: 10.1002/JBM.A.34757
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摘要: Biofilm formation on medical devices is a common cause of implant failure, especially regarding implants that breach the epithelial tissue, so-called transcutaneous implants. Nanotechnology and development new nanomaterials have given opportunity to design nanotextured surfaces. Such surfaces been studied using various in vitro methods showing nanosized features strongly benefit bone cell growth. However, little known how nanostructured affect biofilm formation. The aim this study was therefore examine shape- chemical-dependent effect hydroxyapatite (HA) coating degree Staphylococcus epidermidis Three different types HA particles having shapes calcium phosphate ratios were compared uncoated turned titanium safranin stain assay confocal laser scanning microscopy (CLSM) for assessment biomass bacterial volume, respectively. No difference detected after 6 h incubation with S. epidermidis. Additionally, image analysis CLSM Z-stacks confirmed showed similar results. In conclusion, nanomorphology chemical composition surface coatings did not influence adhesion