Titration of the Histidyl and α-Amino Groups of Hemoglobin

摘要: Abstract The titration data of Antonini, Wyman, Brunori, Fonticelli, Bucci, and Rossi-Fanelli (1), for human horse oxy- deoxyhemoglobins, have been analyzed in a way that yields the number groups which titrate pH region normally assigned to imidazole α-amino groups. results suggest possibility group differs between deoxyproteins above 6.5, is linked alkaline Bohr effect, may be an rather than group, as has assumed heretofore. unavailable groups, arise from histidine residues obliged uncharged state native protein, about 4 per heme hemoglobin 3 hemoglobin. This considerably smaller imidazoles sperm whale myoglobin, also less estimated previous studies.