Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits
作者： Irma Sánchez-Ramos , Ismael Cross , Jaroslav Mácha , Gonzalo Martínez-Rodríguez , Vladimir Krylov
DOI: 10.1100/2012/369802
关键词:
摘要: Growth is a priority trait from the point of view genetic improvement. Molecular markers linked to quantitative loci (QTL) have been regarded as useful for marker-assisted selection in complex traits growth. Polymorphisms studied five candidate genes influencing growth gilthead seabream (Sparus aurata): hormone (GH), insulin-like factor-1 (IGF-1), myostatin (MSTN-1), prolactin (PRL), and somatolactin (SL) genes. Specimens evaluated were commercial broodstock comprising 131 breeders (from which 36 males 44 females contributed progeny). In all samples eleven gene fragments, covering more than 13,000 bp, generated by PCR-RFLP, analyzed; tests made significant associations between these traits. ANOVA results showed association MSTN-1 polymorphism Pairwise revealed several RFLPs with heterogeneity genotypes among size groups. PRL presented linkage disequilibrium. The was mapped centromeric region medium-size acrocentric chromosome pair.
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core.ac.uk参考文章(33)
J. Ruane, B. D. Scherf, V. Martinez, E. P. Guimarães, J. D. Dargie, A. Sonnino, Marker-assisted selection in fish and shellfish breeding schemes. Food and Agriculture Organization of the United Nations (FAO). pp. 329- 362 ,(2007)
Giuseppe Radaelli, Anthea Rowlerson, Francesco Mascarello, Marco Patruno, Bruria Funkenstein, Myostatin precursor is present in several tissues in teleost fish: a comparative immunolocalization study Cell and Tissue Research. ,vol. 311, pp. 239- 250 ,(2003) , 10.1007/S00441-002-0668-Y
Steve Rozen, Helen Skaletsky, Primer3 on the WWW for general users and for biologist programmers. Methods of Molecular Biology. ,vol. 132, pp. 365- 386 ,(2000) , 10.1385/1-59259-192-2:365
C Amirinia, M Bonyadi, RV Torshizi, A Gorbani, A MspI PCR-RFLP within bovin growth hormone gene and its association with sperm quality traits in Iranian Holstein bulls. African Journal of Biotechnology. ,vol. 8, pp. 4811- 4816 ,(2009) , 10.4314/AJB.V8I19.65171
Thomas T. Chen, Adam Marsh, Mike Shamblott, K.-M. Chan, Y.-L. Tang, Clara M. Cheng, B.-Y. Yang, 6 Structure and Evolution of Fish Growth Hormone and Insulinlike Growth Factor Genes Fish Physiology. ,vol. 13, pp. 179- 209 ,(1994) , 10.1016/S1546-5098(08)60067-9
Gonzalo Martínez, Ehwen Monica Shaw, Manuel Carrillo, Silvia Zanuy, Protein Salting-Out Method Applied to Genomic DNA Isolation from Fish Whole Blood BioTechniques. ,vol. 24, pp. 238- 239 ,(1998) , 10.2144/98242BM14
Xiuli Wang, Xiangying Meng, Bin Song, Xuemei Qiu, Haiying Liu, SNPs in the myostatin gene of the mollusk Chlamys farreri: association with growth traits. Comparative Biochemistry and Physiology B. ,vol. 155, pp. 327- 330 ,(2010) , 10.1016/J.CBPB.2009.12.001
Lisa Maccatrozzo, Luca Bargelloni, Barbara Cardazzo, Giorgia Rizzo, Tomaso Patarnello, A novel second myostatin gene is present in teleost fish. FEBS Letters. ,vol. 509, pp. 36- 40 ,(2001) , 10.1016/S0014-5793(01)03124-6
Ismael Cross, Alejandro Merlo, Manuel Manchado, Carlos Infante, José Pedro Cañavate, Laureana Rebordinos, Cytogenetic characterization of the sole Solea senegalensis (Teleostei: Pleuronectiformes: Soleidae): Ag-NOR, (GATA) n , (TTAGGG) n and ribosomal genes by one-color and two-color FISH Genetica. ,vol. 128, pp. 253- 259 ,(2006) , 10.1007/S10709-005-5928-9
Liang Tang, Zhen Yan, Yi Wan, Wei Han, Yingqi Zhang, Myostatin DNA vaccine increases skeletal muscle mass and endurance in mice. Muscle & Nerve. ,vol. 36, pp. 342- 348 ,(2007) , 10.1002/MUS.20791