Synthesis of Zwitterionic Polymer Particles via Combined Distillation Precipitation Polymerization and Click Chemistry for Highly Efficient Enrichment of Glycopeptide

摘要: Because of the low abundance glycopeptide in natural biological samples, methods for efficient and selective enrichment glycopeptides play a significant role mass spectrometry (MS)-based glycoproteomics. In this study, novel kind zwitterionic hydrophilic interaction chromatography polymer particles, namely, poly(N,N-methylenebisacrylamide-co-methacrylic acid)@l-Cys (poly(MBAAm-co-MAA)@l-Cys), was synthesized by facile approach that combined distillation precipitation polymerization (DPP) “thiol–ene” click reaction. DPP approach, residual vinyl groups explored outside core with high density, then functional ligand cysteine immobilized onto surface particles highly thiol–ene Taking advantage unique structure poly(MBAAm-co-MAA)@l-Cys, resulting possess remarkable selectivity from tryptic digested human immunoglobulin G. The ...