Effect of clpP disruption on alkaline sensitivity of Bacillus thuringiensis

摘要: OBJECTIVE We compared alkaline tolerance between Bacillus thuringiensis (Bt) and B. subtilis (Bs) to determine the effect on growth when clpP gene encoding caseinolytic protease is disrupted after shock. METHODS HD73 mutant with deletion of was constructed by homologous recombination. The effects shock, sporulation germination were analyzed. RESULTS Bt can recover from shock medium pH 8.9 9.1 whereas that 8.2 8.4 for Bs. tolerated more than Bs, leading adapt in environment midgut as a pathogen insect. Deletion had no influence germination. grew slower LB addition NaOH 30 mmol/L. It indicates ClpP plays an important role strain.