Coordinate increase in major transcripts from the high pI alpha-amylase multigene family in barley aleurone cells stimulated with gibberellic acid.

摘要: Abstract The purpose of this study was to identify specifically genes and transcripts for the high pI isozyme barley alpha-amylase. From hybridization coding sequence probes blots genomic DNA digested with restriction enzymes that do not cut within our cloned alpha-amylase cDNA, it is estimated about 7 or pseudogenes exist. No difference could be detected between aleurone cell sprout DNAs. Experiments using from 5' 3' untranslated sequences cDNA clone identified three HindIII fragments probably carry sequences. Primer extension experiments used as a primer terminal clone; would cross-hybridize low transcripts. Two major were identified. These shared conserved 23-base immediately ATG start codon, although C----G transversion 3-base deletion present sequence. An unusual 8-base pair GC palindrome in region preceding codon. Distal there no apparent homology. One transcript carrying 97-base identical E. gene other recovered lambda phage library. very similar, but E, demonstrating these arise separate genes. two increased coordinately cells stimulated gibberellic acid. data indicate multigene family at least active members, both which are regulated some manner by plant hormone