Identification and marker-assisted transfer of a new powdery mildew resistance gene at the Pm4 locus in common wheat

作者: Na Li , Haiyan Jia , Zhongxin Kong , Wenbin Tang , Yunxiao Ding

DOI: 10.1007/S11032-017-0670-4

关键词:

摘要: Powdery mildew, a wheat (Triticum aestivum L.) foliar disease caused by Blumeria graminis (DC.) E.O. Speer f. sp. tritici, imposes constant challenge on production in areas with cool or maritime climates. This study was conducted to identify and transfer the resistance gene newly identified common accession ‘D29’. Genetic analysis of F2 population derived from cross D29 susceptible elite cultivar Y158 suggested single dominant is responsible for powdery mildew this germplasm. mapped chromosome 2AL region flanked microsatellite markers Xgdm93 Xhbg327, co-segregated sequence-tagged site (STS) Xsts_bcd1231 TaAetPR5. An allelic test indicated that Pm4 locus. To further evaluate conferred develop new germplasms breeding, gene, as well Pm4a Pm4b, transferred through backcross marker-assisted selection. In spectrum analysis, displayed distinguishable other alleles, including Pm4a, Pm4c, thus designated Pm4e. The identification variation at locus important understanding evolution breeding cultivars resistance.

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