The combined application of nucleotide and amino acid sequences of NS3 hepatitis C virus protein, DNA encoding granulocyte macrophage colony-stimulating factor, and inhibitor of regulatory T cells induces effective immune responce against Hepatitis C virus

摘要: Hepatitis C is related to the most important socially significant human infectious diseases; however, vaccine against this virus up now has notbeen created. One of possible components nonstructural protein NS3 hepatitis (HCV), which synthesized in infected cells and displays protease, NTPase, helicase enzymatic activities. The connection between effectiveness ofT cellular response epitopes spontaneous resolution acute was shown. purpose work compare immune mice inoculation nucleotide amino acid sequences HCV their combination, evaluate adjuvant activity DNA encoding granulocyte macrophage colony-stimulating factor (GM-CSF) influence regulatory T on response. maximum anti-HCV antibody level serum (to 1:640000) induced recombinant rNS3 introduced with aluminum hydroxide. intensive observed after simultaneous administration DNAs full-size GM-CSF. A high lymphocyte proliferation, accumulation IFN-gamma-secreting IFN-gamma, IL-2 release stimulators--NS3 antigens different composition were group mice. It been established that suppression vitro leads statistically increase secretion IFN-gamma. Thus, application along GM-CSF promising approach for development vaccine. expediency inclusion cell inhibitors will be clear special studies.