Cell proliferation and death in the brain of active and hibernating frogs.

摘要: ‘Binomial’ cell proliferation and death have been studied in only a few non-mammalian vertebrates, such as fish. We thought it of interest to map proliferation/apoptosis the brain frog (Rana esculenta L.) this animal species undergoes, during annual cycle, physiological events that could be associated with central nervous system damage. Therefore, we compared active period deep underground hibernation frog. Using western blot analysis for proliferating nuclear antigen (PCNA), revealed positive 36 kDa band all samples found higher optical density values hibernating frogs than frogs. In both frogs, regional differences PCNA-immunoreactive cells terminal transferase dUTP nick-end labelling apoptotic ventricular zones parenchyma areas main encephalon subdivisions. During highest concentration was ventricle dorsal zone cerebral hemispheres but some were apoptotic. By contrast, tectal cerebellar had small or medium amount cells, respectively, number cells. hibernation, an increased observed ventricles This increase primarily evident lateral ventricles, region known ‘hot spot’. Although existed among areas, general being detected optic tectum. particular, these occurred when corresponding zones. suggest high dying parenchymal regions might provide stimulus proliferate. Hibernating utilize neuroprotective strategy face onset neurological damages. hibernator promises valuable model studying mechanisms naturally carried out by order adapt itself survive adverse conditions.