Biochemical changes during reticulocyte maturation in culture. A comparison of genetically different sheep erythrocytes

摘要: A simple culture technique was used to follow sheep reticulocyte maturation. Cells remained in good condition without lysis for at least 7 days and maintained normal intracellular ATP concentrations. Amino acid nucleoside transport were measured during maturation together with changes reduced glutathione (GSH), amino acid, K+ Na+ Reticulocytes of the genetic types high-K+ low-K+, high-GSH low-GSH; nucleoside-permeable nucleoside-impermeable indistinguishable both properties cellular constituents. Cell associated marked decreases L-alanine (Na+-dependent independent), uridine GSH Typical mature-cell levels all parameters reached within period, which time predicted differences between cells different genotypes became apparent. Loss acid-transport activity from low-GSH accompanied by an increase ornithine lysine The rates changed compared.