A comparison of proliferation markers (BrdUrd, Ki-67, PCNA) determined at each cell position in the crypts of normal human colonic mucosa.

摘要: Samples of microscopically normal human sigmoid colon fixed in 70% ethanol from 15 patients who had received bromodeoxyuridine (BrdUrd) prior to surgery have been reanalyzed using a combination proliferation markers. The specimens immunostained for proliferating cell nuclear antigen (PCNA) and after microwave treatment, they stained BrdUrd Ki-67. selected comprised 5 whose mucosa previously gave high labelling indices the crypt, that median values low on previous analysis tissue formal saline different antibody (Potten et al., 1992). relative levels at each position crypts has compared 3 markers with data being as standard S phase cells. One objective was see whether all three discriminated equally well between groups patient samples. show distinction high, medium seen retained when Ki-67 immunostaining analysed. PCNA resulted were largely lost.