High atomic weight, high-energy radiation (HZE) induces transcriptional responses shared with conventional stresses in addition to a core "DSB" response specific to clastogenic treatments.

摘要: Plants exhibit a robust transcriptional response to gamma radiation which includes the induction of transcripts required for homologous recombination and suppression that promote cell cycle progression. Various DNA damaging agents induce different spectra damage as well "collateral" other cellular components therefore are not expected provoke identical responses by cell. Here we study effects two types ionizing (IR) treatment, HZE (1 GeV Fe(26+) high mass, charge, energy relativistic particles) photons, on transcriptome Arabidopsis thaliana seedlings. Both IR small clusters radicals can result in formation double strand breaks (DSBs), but also produces linear arrays extremely clustered damage. We performed these experiments across range time points (1.5-24 h after irradiation) both wild-type plants mutants defective DSB-sensing protein kinase ATM. The shared break-repair-related response, although they differ slightly timing, degree, ATM-dependence response. ATM-dependent, metabolism-related "DSB response" were induced agents, conventional stresses. Gamma irradiation induced, at 24 post-irradiation, ATM-dependent associated with variety stresses; overrepresented pathogen rather than metabolism. In contrast, only HZE-irradiated plants, 1.5 irradiation, exhibited an additional very extensive experiencing "extended night." This was apparent gamma-irradiated plants.