Analysis of the biologic functions of H- and L-ferritins in HeLa cells by transfection with siRNAs and cDNAs: evidence for a proliferative role of L-ferritin.
作者： Anna Cozzi , Barbara Corsi , Sonia Levi , Paolo Santambrogio , Giorgio Biasiotto
DOI: 10.1182/BLOOD-2003-06-1842
关键词:
摘要: We describe the use of small interfering RNAs (siRNAs) to down-regulate H- and L-ferritin levels in HeLa cells. siRNAs repressed expression about 20% 25% background level both stable transient transfections. cells transfected with cDNAs were analyzed parallel compare effects ferritin up- down-regulation. found that large modifications did not affect iron availability but positively affected cell proliferation rate an iron-independent manner. The down-regulation H-ferritin modified cellular resistance oxidative damage, as expected. In contrast, suppression clones increase made less resistant supplementation chelation. results indicate has no direct on homeostasis cells, while it new, iron-unrelated functions. addition, they suggest function is act buffer.
ashpublications.org
elsevier.com
bloodjournal.org参考文章(43)
Paolo Arosio, Sonia Levi, Ferritin, iron homeostasis, and oxidative damage1,2 1Guest Editor: Mario Comporti 2This article is part of a series of reviews on “Iron and Cellular Redox Status.” The full list of papers may be found on the homepage of the journal. Free Radical Biology and Medicine. ,vol. 33, pp. 457- 463 ,(2002) , 10.1016/S0891-5849(02)00842-0
Theil Ec, The IRE (iron regulatory element) family: structures which regulate mRNA translation or stability. Biofactors. ,vol. 4, pp. 87- 93 ,(1993)
K White, H N Munro, Induction of ferritin subunit synthesis by iron is regulated at both the transcriptional and translational levels. Journal of Biological Chemistry. ,vol. 263, pp. 8938- 8942 ,(1988) , 10.1016/S0021-9258(18)68398-X
Sonia Levi, Paolo Santambrogio, Anna Cozzi, Ermanna Rovida, Barbara Corsi, Elena Tamborini, Stefania Spada, Alberto Albertini, Paolo Arosio, The role of the L-chain in ferritin iron incorporation. Studies of homo and heteropolymers. Journal of Molecular Biology. ,vol. 238, pp. 649- 654 ,(1994) , 10.1006/JMBI.1994.1325
Andrew R.J. Curtis, Constanze Fey, Christopher M Morris, Laurence A. Bindoff, Paul G. Ince, Patrick F. Chinnery, Alan Coulthard, Margaret J. Jackson, Andrew P. Jackson, Duncan P. McHale, David Hay, William A. Barker, Alex F. Markham, David Bates, Ann Curtis, John Burn, Mutation in the gene encoding ferritin light polypeptide causes dominant adult-onset basal ganglia disease. Nature Genetics. ,vol. 28, pp. 350- 354 ,(2001) , 10.1038/NG571
Elizabeth C. Theil, Ferritin: At the Crossroads of Iron and Oxygen Metabolism Journal of Nutrition. ,vol. 133, ,(2003) , 10.1093/JN/133.5.1549S
Kristen S. Kulp, Sherril L. Green, P.Richard Vulliet, Iron Deprivation Inhibits Cyclin-Dependent Kinase Activity and Decreases Cyclin D/CDK4 Protein Levels in Asynchronous MDA-MB-453 Human Breast Cancer Cells Experimental Cell Research. ,vol. 229, pp. 60- 68 ,(1996) , 10.1006/EXCR.1996.0343
Eunice L. Kwak, Denis A. Larochelle, Carole Beaumont, Suzy V. Torti, Frank M. Torti, Role for NF-κB in the Regulation of Ferritin H by Tumor Necrosis Factor-α Journal of Biological Chemistry. ,vol. 270, pp. 15285- 15293 ,(1995) , 10.1074/JBC.270.25.15285
Or KAKHLON, Yosef GRUENBAUM, Z. Ioav CABANTCHIK, Ferritin expression modulates cell cycle dynamics and cell responsiveness to H-ras-induced growth via expansion of the labile iron pool. Biochemical Journal. ,vol. 363, pp. 431- 436 ,(2002) , 10.1042/0264-6021:3630431
Sonia Levi, Barbara Corsi, Marta Bosisio, Rosangela Invernizzi, Armin Volz, David Sanford, Paolo Arosio, Jim Drysdale, A Human Mitochondrial Ferritin Encoded by an Intronless Gene Journal of Biological Chemistry. ,vol. 276, pp. 24437- 24440 ,(2001) , 10.1074/JBC.C100141200