The singlet-oxygen-sensitized delayed fluorescence in mammalian cells: a time-resolved microscopy approach
作者： Marek Scholz , Anna-Louisa Biehl , Roman Dědic , Jan Hála
DOI: 10.1039/C4PP00339J
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摘要: The present work provides a proof-of-concept that the singlet oxygen-sensitized delayed fluorescence (SOSDF) can be detected from individual living mammalian cells in time-resolved microscopy experiment. To this end, 3T3 mouse fibroblasts incubated with 100 μM TPPS4 or TMPyP were used and microsecond kinetics of (DF) recorded. analysis revealed SOSDF is major component overall DF signal. approach enables precise control experimental conditions – are clearly influenced by presence 1O2 quencher (sodium azide), H2O/D2O exchange, oxygen concentration. Analysis kinetics, which was reconstructed as difference between unquenched NaN3-quenched samples, cellular lifetime τΔ = 1–2 μs triplet τT 22 ± 5 agreement previously published values. short acquisition times, typically range tens seconds, enable us to study dynamic processes. It shown lifetimes increase during PDT-like treatment, may provide valuable information about changes intracellular microenvironment. proposed evaluated an alternative tool for detection biological systems.
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