Depthwise microbiome and isotopic profiling of a moderately saline microbial mat in a solar saltern.

摘要: The solar salterns in Tuticorin, India, are man-made, saline to hypersaline systems hosting some uniquely adapted populations of microorganisms and eukaryotic algae that have not been fully characterized. Two visually different microbial mats (termed ‘white’ ‘green’) developing on the reservoir ponds (53 PSU) were isolated from salterns. Firstly, archaeal bacterial diversity vertical layers analyzed. Culture-independent 16S rRNA gene analysis revealed both bacteria archaea rich their diversity. The top had a higher representation halophilic Halobacteriaceae, phylum Chloroflexi, classes Anaerolineae, Delta- Gamma- Proteobacteria than deeper sections, indicating salinity gradient exists within mats. Limited presence Cyanobacteria detection algae-associated bacteria, such as Phycisphaerae, Phaeodactylibacter Oceanicaulis likely implied other phototrophs could be primary producers mat ecosystem. Secondly, predictive metabolic pathway using data addition regulatory functions, methane nitrogen metabolisms prevalent. Finally, stable carbon isotopic compositions determined samples showed δ13Corg δ15Norg values increased slightly with depth, ranging − 16.42 14.73‰, 11.17 13.55‰, respectively. signature along profile followed pattern is distinctive community composition net activities, comparable results discussions presented here by merging culture-independent studies, analyses characterization, provide collective strategy understand compositional functional characteristics environments.