ETV4 plays a role on the primary events during the adenoma-adenocarcinoma progression in colorectal cancer.

摘要: Colorectal cancer (CRC) is one of the most common cancers worldwide; it fourth leading cause death in world and third Brazil. Mutations APC, DCC, KRAS TP53 genes have been associated with progression sporadic CRC, occurring at defined pathological stages tumor consequently modulating several corresponding signaling pathways. Therefore, identification gene signatures that occur each stage during CRC critical can present an impact on diagnosis prognosis patient. In this study, our main goal was to determine these signatures, by evaluating expression paired colorectal adenoma adenocarcinoma samples identify novel genetic markers association adenoma-adenocarcinoma transition. Ten were subjected microarray analysis. addition, mutations investigated DNA sequencing adenoma, adenocarcinoma, normal tissue, peripheral blood from ten patients. Gene analysis revealed a signature 689 differentially expressed (DEG) (fold-change> 2, p< 0.05), between analyzed. pathway using DEG identified important pathways such as remodeling extracellular matrix epithelial-mesenchymal Among DEG, ETV4 stood out samples, further confirmed set TCGA database. Subsequent vitro siRNA assays against resulted decrease cell proliferation, colony formation migration HT29 SW480 lines. pathogenic mutations, exclusively adenocarcinomas samples. Our study high potential be used biomarkers special emphasis gene, which demonstrated involvement proliferation migration.