The SMC-Like Protein Complex SbcCD Enhances DNA Polymerase IV-Dependent Spontaneous Mutation in Escherichia coli

摘要: In Escherichia coli, RpoS, the general stress response sigma factor, regulates activity of specialized DNA polymerase IV (Pol IV) both in stationary-phase and exponential-phase cells. Because during exponential phase dinB, gene encoding Pol IV, is transcribed independently RpoS must regulate growing cells indirectly via one or more intermediate factors. The results presented here show that these factors SbcCD, an SMC-like protein ATP-dependent nuclease. By initiating participating double-strand break repair, SbcCD may provide substrates for activity.