Modulation of Polyketide Synthase Activity by Accessory Proteins During Lovastatin Biosynthesis
作者： Jonathan Kennedy , Karine Auclair , Steven G Kendrew , Cheonseok Park , John C Vederas
DOI: 10.1126/SCIENCE.284.5418.1368
关键词:
摘要: Polyketides, the ubiquitous products of secondary metabolism in microorganisms, are made by a process resembling fatty acid biosynthesis that allows suppression reduction or dehydration reactions at specific biosynthetic steps, giving rise to wide range often medically useful products. The lovastatin cluster contains two type I polyketide synthase genes. Synthesis main nonaketide-derived skeleton was found require previously known iterative nonaketide (LNKS), plus least one additional protein (LovC) interacts with LNKS and is necessary for correct processing growing chain production dihydromonacolin L. noniterative diketide (LDKS) enzyme specifies formation 2-methylbutyrate closely an transesterase (LovD) responsible assembling from this monacolin J.
sciencemag.org
harvard.edu参考文章(22)
D L Rainwater, P E Kolattukudy, Fatty acid biosynthesis in Mycobacterium tuberculosis var. bovis Bacillus Calmette-Guérin. Purification and characterization of a novel fatty acid synthase, mycocerosic acid synthase, which elongates n-fatty acyl-CoA with methylmalonyl-CoA. Journal of Biological Chemistry. ,vol. 260, pp. 616- 623 ,(1985) , 10.1016/S0021-9258(18)89777-0
Stuart Smith, The animal fatty acid synthase: one gene, one polypeptide, seven enzymes. The FASEB Journal. ,vol. 8, pp. 1248- 1259 ,(1994) , 10.1096/FASEBJ.8.15.8001737
Jean K. Chan, Richard N. Moore, Thomas T. Nakashima, John C. Vederas, Biosynthesis of mevinolin. Spectral assignment by double-quantum coherence NMR after high carbon-13 incorporation Journal of the American Chemical Society. ,vol. 105, pp. 3334- 3336 ,(1983) , 10.1021/JA00348A062
L. R. TREIBER, R. A. REAMER, C. S. ROONEY, H. G. RAMJIT, Origin of monacolin L from Aspergillus terreus cultures. The Journal of Antibiotics. ,vol. 42, pp. 30- 36 ,(1989) , 10.7164/ANTIBIOTICS.42.30
Y. Kono, J.M. Daly, Characterization of the host-specific pathotoxin produced by Helminthosporium maydis, race T, affecting corn with Texas male sterile cytoplasm Bioorganic Chemistry. ,vol. 8, pp. 391- 397 ,(1979) , 10.1016/0045-2068(79)90064-6
David J. Witter, John C. Vederas, Putative Diels−Alder-Catalyzed Cyclization during the Biosynthesis of Lovastatin Journal of Organic Chemistry. ,vol. 61, pp. 2613- 2623 ,(1996) , 10.1021/JO952117P
PE Kolattukudy, Norvin D Fernandes, AK Azad, Ann Marie Fitzmaurice, Tatiana D Sirakova, None, Biochemistry and molecular genetics of cell-wall lipid biosynthesis in mycobacteria Molecular Microbiology. ,vol. 24, pp. 263- 270 ,(1997) , 10.1046/J.1365-2958.1997.3361705.X
Eric Guenzi, Giuliano Galli, Ingeborg Grgurina, Dennis C. Gross, Guido Grandi, Characterization of the Syringomycin Synthetase Gene Cluster A LINK BETWEEN PROKARYOTIC AND EUKARYOTIC PEPTIDE SYNTHETASES Journal of Biological Chemistry. ,vol. 273, pp. 32857- 32863 ,(1998) , 10.1074/JBC.273.49.32857
S Donadio, M. Staver, J. McAlpine, S. Swanson, L Katz, Modular organization of genes required for complex polyketide biosynthesis. Science. ,vol. 252, pp. 675- 679 ,(1991) , 10.1126/SCIENCE.2024119
David E Cane, Christopher T Walsh, Chaitan Khosla, Harnessing the Biosynthetic Code: Combinations, Permutations, and Mutations Science. ,vol. 282, pp. 63- 68 ,(1998) , 10.1126/SCIENCE.282.5386.63