Distribution of the intermediate elements operating in ER to Golgi transport

摘要: We have used a 58 kDa membrane protein (p58) as marker to study the transport pathway between rough endoplasmic reticulum (ER) and Golgi apparatus. Immunolocalization of p58 in fibroblasts showed its presence single cisterna small tubular vesicular elements at cis side In addition, was detected large (200–500 nm diameter) tubulovesicular structures, clustered region but also found peripheral locations. These represent intermediates ER since they contained newly synthesized viral glycoproteins, arrested cells 15 degrees C. The structures accumulated low temperature reclustered rapidly upon shift back 37 This movement involved long intracellular distances efficiently inhibited by nocodazole, indicating that it requires integrity microtubules. contrast, reclustering unaffected brefeldin A (BFA), suggesting this compound affects prior temperature-sensitive step. BFA-treated localized scattered, tubular, smooth clusters, close association with cisternae. cellular distribution intermediate indicates sites exit are widely distributed within network. suggest locations where accumulates could such sites.