Fine mapping of QTL and microarray gene expression studies in arabidopsis using STAIRS

摘要: QTL mapping with segregating populations results in poor map resolution which limits the applicability of mapped further research such as gene cloning. The current project aimed mainly at developing STepped Aligned Recombinant Inbred Strains (STAIRS) covering top region chromosome 3 and demonstrating feasibility using STAIRS high Arabidopsis. Arabidopsis had been reported to house related flowering time. This was first saturated 24 polymorphic microsatellite markers 23 narrow were produced within via a marker-assisted backcross breeding programme whole substitution lines. analysis revealed major pleiotropic 2-3 cM affecting time, leaf number day 20 rosette cauline numbers flowering. A second less but opposite effect on same traits located 15-20 cM. search for candidate genes 3, locate possible COL-2, CONSTANS-Like affects Microarray expression profiling performed two genotypically closest lines differ time compare chronological physiological ages experiments respectively. lists differentially expressed obtained from experiments. Differential observed latter experiment. emphasized power fine possibility them transcriptional study genes.