Characteristics of microcystin production in the cell cycle of Microcystis viridis.
作者： Keishi Kameyama , Norio Sugiura , Yuhei Inamori , Takaaki Maekawa
DOI: 10.1002/TOX.10147
关键词:
摘要: The correlation between the content of three microcystins (types LR, RR and YR) cell cycle an axenic strain Microcystis viridis, NIES-102, was investigated under conditions high (16 mg L(-1)) low (1.0 nitrate (NO(3)-N) concentrations. Each phase identified using a flow cytometer equipped with 488-nm argon laser SYTOX Green dye, which binds specifically to nucleic acids can be exited by wavelength (Ex/Em: 504/523 nm on DNA). Microcystin concentration showed positive linear DNA concentration. microcystin cells changed remarkably as process proceeded, maximum in G(2)/M minimum G(0)/G(1) phase. Under condition NO(3)-N concentration, ratio total that about 6:1. In contrast, for two batch cultures 1.3-fold greater compositions also along process, although there little difference composition related Therefore, were distinctive specific each cycle, M. viridis more strongly responsible both quantity types production than effect
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