Identification by suppression subtractive hybridization and expression analysis of Medicago truncatula putative defence genes in response to Orobanche crenata parasitization
作者： José Vicente Die , Miguel A. Dita , Franziska Krajinski , Clara I. González-Verdejo , Diego Rubiales
DOI: 10.1016/J.PMPP.2007.06.001
关键词:
摘要: Crenate broomrape (Orobanche crenata) is the major constraint for pea and faba bean production in Mediterranean region. In this study, a systematic sequencing of expressed sequence tags (ESTs) was chosen to obtain first global picture assembly genes involved defence response. A cDNA-library established by suppression subtractive hybridization model legume Medicago truncatula infected O. crenata order identify large number host plant ESTs. Eighty-one presumably up-regulated were identified classified functional categories. EST-annotations showed homologies well-characterized genes. Most proteins encoded these genes, are already known M. truncatula, such as related JA pathway or cell wall modifications. notable ESTs, however, derived from novel not matching entries large-scale sequences collections. Expression analyses quantitative RT-PCR 20 corresponding different categories high expression levels, supporting their involvement
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