[44] Monoterpene and sesquiterpene cyclases

摘要: Publisher Summary A detailed description of the procedures that have been used for degradation terpenoid metabolites is scope this chapter. However, routes determining location label in camphor, fenchone, a-terpinene, 7-terpinene, limonene, cineole, and a- fl-pinene from 1-SH-labeled acyclic precursors are described Similarly, a number sesquiterpenes derived specifically labeled [SH]- [14C]farnesyl pyrophosphate other described. Assay cyclase activity crude extracts generally unreliable due to great dilution enzyme and/or high phosphate concentration presence inhibitory substances. Preliminary protein adjustment more suitable assay conditions required. For large-scale preparations, ultrafiltration (Amicon PM 30) desalting with buffer change on Sephadex G-25 columns preferred speed. small-scale dry G- 25-300 very effective. The polyols thiol reagents also markedly improve stability during purification.