Hydrogen bonding of cholesterol in the lipidic cubic phase.

摘要: The addition of cholesterol to the monoolein-based lipidic cubic phase (LCP) has been instrumental in obtaining high-resolution crystal structures several G protein-coupled receptors. Here, we report use magic angle spinning NMR spectroscopy record and assign isotropic 13C chemical shifts lamellar phases at different hydration levels with monoolein chain-deuterated DMPC as host lipids. hydrogen-bonding patterns these were determined from data by quantum calculations. results are consistent normal orientation lipid bilayers hydroxyl group located hydrophobic/hydrophilic interface. mostly affected identity little or no dependency on (20% vs 40%) (lamellar LCP). In bilayers, choleste...