Test sample allergenicity detection method and kit thereof

摘要: The invention relates to a test sample allergenicity detection method and kit thereof. comprises the following steps: randomly dividing plurality of mice into groups; measuring thickness ears each mouse different entering first time period; within period, smear an equal amount reagent on second when period is ended, firstly thicknesses groups, further killing mice, taking down lymph glands after under sterile condition, preparing cell suspension, putting 5-bromodeoxyuridine solution culturing, testing content the5-bromodeoxyuridine by using ELISA (Enzyme-Linked Immuno Sorbent Assay) method; judging substance according limiting standards. By adopting method, whether has positive can be tested, intraperitoneal injection avoided, then animals prevented from pain, greatly reduced,and cost reduced.