Quantitative studies of in situ immune complex glomerulonephritis in the rat induced by planted, cationized antigen

摘要: Cationized human IgG can bind to the rat glomerular basement membrane (GBM), act as planted antigen, and induce in situ immune complex formation accompanied by severe glomerulonephritis. Perfusion of highly cationized (isoelectric point {more than} 9.5) via left renal artery resulted preferential localization within perfused kidney (up 56 percent dose injected); after intravenous administration, only 4 was bound kidneys. The antigen localized along capillary walls accessible for antibody administered intravenously 1 h perfusion, when virtually no remained circulation. Persistence markedly prolonged complexed with antibody; one-half still present 12 d. There a difference disappearance rates antibody, removed faster from than binding which almost unchanged during first week. Renal perfusion minimum 20 mug IgG, followed injection regularly induced glomerulonephritis proteinuria at least 100 mg/24 h. degree persistence depended on perfused. Experiments using radiolabeled showed that 11.1 time injection. At onset proteinuria, 4.0 31.9 anti-human were kidney. Immunofluorescence revealed deposits consisting rabbit (anti-human IgG) GBM. staining pattern linear (confluent) 2 d became granular course disease. Electronmicroscopically, prominent finding accumulation dense deposits, mainly subepithelial space beneath slit pores.