Cholesterol Esterase produced by Streptomyces lavendulae. II. Purification and Properties as a Lipolytic Enzyme

摘要: Cholesterol esterase was purified about 85 times from the broth filtrate of Streptomyces lavendulae by procedures involving precipitation with ammonium sulfate, reprecipitation acetone, affinity chromatography on palmitoyl cellulose, and ion exchange DEAE-cellulose. The enzyme showed a single band polyacrylamide disc gel electrophoresis SDS-polyacrylamide electrophoresis, specific activity 36.3 U/mg. molecular weight 12000 as determined electrophoresis. different properties other cholesterol esterases regards its for cholesterol.