An Ethylene-responsive Factor BpERF11 Negatively Modulates Salt and Osmotic Tolerance in Betula platyphylla

摘要: Ethylene responsive factors (ERFs) play important roles in the abiotic stress; however, only a few ERF genes from woody plants have been functionally characterized. In present study, an gene Betula platyphylla (birch), BpERF11, was characterized response to stress. BpERF11 is nuclear protein, which could specifically bind GCC boxes and DRE motifs. BpERF11-overexpressing RNA interference (RNAi) knockdown were generated for gain- loss-of-function analysis. negatively regulates resistance salt severe osmotic stress, transgenic birch overexpressing shows increased electrolyte leakage malondialdehyde (MDA) contents. inhibits expression of AtMYB61 homologous gene, resulting stomatal aperture, elevated transpiration rate. Furthermore, downregulates P5CS, SOD POD genes, but upregulates PRODH P5CDH, results reduced proline levels reactive oxygen species (ROS) accumulation. also significantly LEA dehydrin that involve stress tolerance. Therefore, serves as transcription factor tolerance by modulating various physiological processes.