Cytokines cause cultured retinal pigment epithelial cells to secrete metalloproteinases and to contract collagen gels.

摘要: PURPOSE Because retinal pigment epithelial cells in epiretinal membranes remodel and contract their surrounding extracellular matrix, investigations were performed to determine if these can produce matrix metalloproteinases collagen gels vitro the presence of serum or cytokines. METHODS Cells grown on production was measured using zymography. RESULTS a three-dimensional gel culture system several latent that are secreted into medium. These include molecules 49, 56, 66, 100 kD. In addition, an enzyme is probably active form 66 kD present. When interleukin 1 beta added cultures, 49 gelatinase greatly stimulated both enzymes also observed contrast, transforming growth factor has no stimulatory effect. The but this small without cytokines; however, contraction enhanced plus beta. Contraction unlikely be result metalloproteinase action underlying because complete inhibition little CONCLUSIONS results show cytokines cause cultured can, when activated, degrade most collagens other structural matrix. they stimulate constituents there not simple causal relationship between remodeling contraction.