Kainate Receptor-Mediated Responses in the CA1 Field of Wild-Type and GluR6-Deficient Mice

摘要: Kainate receptors are abundantly expressed in the hippocampus. Mice with disruption of kainate receptor subunits allow genetic dissection role each synaptic physiology hippocampus, as well excitotoxic processes. We have compared action domoate and on CA1 pyramidal neurons slices from wild-type GluR6−/− mice. The difference amplitude inward currents evoked by between mice demonstrates presence functional neurons. Block domoate-activated AMPA antagonists 2,3-dihydroxy-6-nitro-7-sulfonyl-benzo(F)quinoxaline (1 μm) 1-(4-aminophenyl)-3-methylcarbamyl-4-methyl7,8-methylenedioxy-3,4-dihydro-5H-2,3-benzodiazepine) (GYKI 53655) (50 is complete but only partial In GYKI 53655, activation dramatically increases frequency spontaneous IPSCs cells wild-type, GluR6−/−, This results receptor-mediated a sustained current an increased potential firing afferent GABAergic interneurons field. These effects observed also decrease increasing failures indicate that cells, distinct subtypes mediate several functionally antagonistic effects.