RNase P-mediated inhibition of cytomegalovirus protease expression and viral DNA encapsidation by oligonucleotide external guide sequences.

摘要: External guide sequences (EGSs) are oligonucleotides that consist of a sequence complementary to target mRNA and recruit intracellular RNase P for specific degradation the RNA. In this study, DNA-based EGS molecules were chemically synthesized coding protease human cytomegalovirus (HCMV). The efficiently directed cleave in vitro. When EGSs exogenously administered into HCMV-infected foreskin fibroblasts, reduction about 80–90% expression level 300-fold HCMV growth observed cells treated with functional EGS, but not or “disabled” carrying nucleotide mutations precluded recognition. Moreover, packaging viral DNA genome capsid was blocked EGS. These results indicate is essential encapsidation. our study provides direct evidence exogenous administration can be used as therapeutic approach inhibiting gene replication virus.