Means and method for enhancing nucleic acid hybridization
作者： James John Hogan , Curt Lawrence Milliman
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摘要: The binding of a nucleic acid probe with its complementary sequence in targeted, single stranded is affected by the secondary and tertiary structure target acid. rate extent hybridization targeted can be increased use "helper" oligonucleotides. Helper oligonucleotides are selected to bind impose different on facilitate target. resulting hybrid also exhibits higher T m than which results from addition alone.
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