New methods for gamete rescue from gonads of nondomestic felids.

摘要: Methods for rescuing oocytes and spermatozoa post mortem are described, which were adapted from domestic cat as a model. Ovaries mechanically processed large-scale recovery of oocytes. Numbers intact cumulus-oocyte complexes (COCs) per animal preantral follicles ovary 18 +/- 2 2892 665 in cats, respectively. Similar results obtained 13 individuals 6 nondomestic felids: 16 COCs 1867 1144 follicles. Preantral cultured at least 7 days. Intact maturated 24 h fertilized vitro with homologous or heterologous (from cat) spermatozoa. Spermatozoa collected caudae epididymides (n = 11; five species) cryopreserved 8) using programmed freezer. The reproductive competence was demonstrated by development to embryos (> 8 cells) vitro. Spermatogenic efficiency males assessed flow cytometric analysis mitotic meiotic testicular cells well estimation testosterone concentration testes. demonstrate the possibility retrospective assessment male female capacity. In conclusion, described methods could be useful part gamete rescue programmes endangered felids.