In vivo small animal imaging for early assessment of therapeutic efficacy of photodynamic therapy for prostate cancer

摘要: We are developing in vivo small animal imaging techniques that can measure early effects of photodynamic therapy (PDT) for prostate cancer. PDT is an emerging therapeutic modality continues to show promise the treatment of At our institution, a new second-generation photosensitizing drug, silicon phthalocyanine Pc 4, has been developed and evaluated at Case Comprehensive Cancer Center. In this study, we magnetic resonance (MRI) provide therapy monitoring assessment tumor response PDT. We generated human cancer xenografts athymic nude mice. For experiments, used highfield 9.4-T MR scanner (Bruker Biospec). High-resolution images were acquired from treated and control tumors pre- post-PDT 24 hr after PDT. utilized multi-slice multi-echo (MSME) MR sequences. During acquisitions, animals anesthetized with continuous supply 2% isoflurane in oxygen continuously monitored respiration temperature. After manually segmented on each image slice quantitative analyses. computed three-dimensional T2 maps for regions MSME images. plotted histograms maps and post-PDT dissected tissues the histologic slides validate six mice imaged and treated Center Imaging Research. The values increased by ± 14% hr after therapy. did not demonstrate significant changes values. Inflammation and necrosis observed within hour treatment. Preliminary results 4-PDT is effective treatment provides useful tool to evaluate