Use of regulated cell lysis in a lethal genetic selection in Escherichia coli: identification of the autoinducer-binding region of the LuxR protein from Vibrio fischeri ATCC 7744.

作者: G S Shadel , R Young , T O Baldwin

DOI: 10.1128/JB.172.7.3980-3987.1990

关键词:

摘要: A lethal genetic selection utilizing the bacteriophage lambda lysis genes (S, R, RZ) has been developed and used in conjunction with a luminescence screen to allow isolation characterization of six missense mutations two nonsense luxR gene from Vibrio fischeri ATCC 7744. transcriptional fusion operonR downstream truncated luxI allows control cell by addition synthetic autoinducer growth medium. The isolated resulted changes LuxR protein Asp at position 79 Asn (hereafter designated as D79N), V82I, V109L, L118F, S123I, H217Y. Variant proteins amino acid D79N, V82L, H127Y were shown require higher concentrations elicit certain amplitude response than is required wild-type protein. We believe that clustering total seven randomly generated 49-amino-acid region primary sequence defines critical portion observation lesions this responded elevated levels suggests autoinducer-binding site constructed, least part, several residues within 79-to-127

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