Analysis of the promoter of an abscisic acid responsive late embryogenesis abundant gene of Arabidopsis thaliana

摘要: Abstract Late embryogenesis abundant (lea) proteins are a diverse group of present in many mono- and dicotyledonous plants. The genes encoding lea expressed the embryo during late stages seed development. However, expression can also be induced immature seeds vegetative tissues by abscisic acid (ABA). Lea thus provide model with which to study tissue-specific, developmental hormonal regulation expression. We used β-glucuronidase ( iudA ) reporter gene (gus) identify functional domains promoter Arabidopsis thaliana (AtEm1). found that fragment extending from −182 bp +72 is sufficient direct gus embryos pollen transgenic tobacco. Gus was developmentally regulated, being anther Comparison different deletion constructs showed both sequences between −1443 −430 had no effect on level expression, whilst region necessary for full response ABA studied seedlings tobacco transformed fusion construct containing promoter. Treatment 50 μM resulted 3–4-fold increase GUS activity, indicating induction AtEm1 acts at least part transcription. Conserved regulatory elements were identified sequence analysis. possible role these elements, significance observed discussed.