Characterization and expression profile of Vitellogenin gene from Scylla paramamosain

摘要: Abstract The full-length (7816 bp) cDNA of Vitellogenin (Vtg) encoding 2560 aa with an estimated molecular mass 287.743 kDa was cloned from the green mud crab Scylla paramamosain. Semi-quantitative PCR (sq-PCR) revealed a specific expression pattern Sp-vtg gene in ovaries and hepatopancreas. With development ovaries, level showed increasing trend both hepatopancreas, hepatopancreas ovary stable after stage IV. By situ hybridization, positive signals were detected cytoplasm oocytes I, follicle cell surrounding nucleus III, V. Furthermore, become stronger later stages ovary. Moreover, hybridization analysis that are present hepatopancreatic tubule, increase during development, becoming strongest Our results indicate sites synthesis S.