The Effect of Lactobacillus acidophilus PTCC 1643 on Cultured Intestinal Epithelial Cells Infected with Salmonella enterica serovar Enteritidis.

摘要: Objectives Gastrointestinal disorders caused by Salmonella enterica serovar Enteritidis (SesE) are a significant health problem around the globe. Probiotic bacteria have been shown to positive effects on immune responses. Lactobacillus acidophilus was examined for its capability influence innate response of HT29 intestinal epithelial cells towards SesE. The purpose this work assess effect L. PTCC 1643 cultured infected with Methods were in Roswell Park Memorial Institute medium supplemented 10% fetal bovine serum and 1% penicillin/streptomycin. treated after or before challenge At 2 4 hours post-infection, we measured changes expression levels TLR2 TLR4 via real-time polymerase chain reaction. Results Treatment inhibited SesE-induced increases cells. Moreover, that pretreated then SesE significantly higher than without pretreatment. Taken together, results indicated had an anti-inflammatory modulated influencing expression. Conclusion Our findings suggested able suppress inflammation infection reduce Additional vivo vitro studies required further elucidate mechanisms underlying effect.