Toll-like receptor-dependent IL-12 production by dendritic cells is required for activation of natural killer cell-mediated Type-1 immunity induced by Chrysanthemum coronarium L.

摘要: Type-1 immunity has an essential role for our host defenses against cancer and outer pathogens such as bacteria virus. We demonstrated here that the edible plant extract of Chrysanthemum coronarium L. (C. coronarium) remarkably activates in a Toll-like receptor (TLR)2-, TLR4-, TLR9-dependent manner. In present experiments, C. significantly induces interferon (IFN)-γ production by mouse spleen cells. addition, IFN-γ cells was completely blocked addition anti-Interleukin (IL)-12 monoclonal antibodies. confirmed NK1.1(+) natural killer (NK) cells, NKT CD11c(+) dendritic (DC) were immediately activated after stimulation with abolished cell-depleted The caused DC maturation involving up-regulations surface expression levels MHC class I, II, CD40, CD86 well induction IL-12 production. induced reduced depleted Furthermore, strongly TLR4- partially TLR2- TLR9-deficient Thus, we cellular mechanism activation via NK coronarium. These findings suggest would be promising immuno-improving adjuvant, which might useful prevention infectious, cancer, allergic diseases through immunity.