Needle-based sampling coupled with colorimetric reaction catalyzed by layered double hydroxide peroxidase mimic for rapid detection of the change of d-glucose levels with time in bananas.

摘要: Abstract For analyte detection in raw fruits, the conventional sample pretreatment method usually involves mashing (blending or homogenization), extraction, and dilution. This process is time-consuming, solvent-intensive laborious. Usually, there also a lot wastage with multiple fruits being combined into composite samples. In this work, new micro-sampling approach based on syringe needle developed; it was coupled micro liquid-phase extraction to determine quantify d -glucose bananas. sampling quick, easy required only minimal use of solvent. The extracted banana flesh first oxidized enzymatically glucose oxidase. resulting peroxide then detected colorimetrically via oxidation 3,3′,5,5′-tetramethylbenzidine presence catalyst. latter, consisting an iron (III)–nickel (II) layered double hydroxide (LDH[NiII-FeIII]) synthesized work for purpose. results provided low limits (0.025 μg/mL), wide linear range (0.1–3000 μg/mL) good linearity (r2 = 0.9998). Quantification using applied samples over period 10 days. showed that levels bananas increased as ripened, expected. demonstrated interesting efficient analytes fruit