Exosomes from Plasmodium yoelii-Infected Reticulocytes Protect Mice from Lethal Infections

摘要: Exosomes are 30–100-nm membrane vesicles of endocytic origin that released after the fusion multivesicular bodies (MVBs) with plasma membrane. While initial studies suggested role exosomes was limited to removal proteins during maturation reticulocytes erythrocytes, recent indicate they produced by different types cells and involved in promoting inter-cellular communication antigen presentation. Here, we describe isolation characterization from peripheral blood BALB/c mice infected reticulocyte-prone non-lethal Plasmodium yoelii 17X strain. Importantly, proteomic analysis revealed presence parasite these vesicles. Moreover, immunization purified elicited IgG antibodies capable recognizing P. yoelii-infected red cells. Furthermore, lethal challenge immunized normocyte-prone 17XL strain caused a significant attenuation course parasitaemia, increased survival time, altered cell tropism reticulocytes. These results were obtained also when isolated reticulocyte culture indicating reticulocyte-derived carry antigens immune modulation. inclusion CpG ODN 1826 exosome immunizations IgG2a IgG2b promoted survival, clearance parasites subsequent sterile protection 83% animals challenged yoelli 17XL. To our knowledge, this is first report responses derived opening new avenues for modulation anti-malaria responses.