Rapamycin relieves inflammation of experimental autoimmune encephalomyelitis by altering the balance of Treg/Th17 in a mouse model.
作者： Zhenfei Li , lingling Nie , Liping Chen , Yafei Sun , Guo Li
DOI: 10.1016/J.NEULET.2019.04.035
关键词:
摘要: Abstract This study was to observed the different doses of rapamycin on treatment experimental autoimmune encephalomyelitis (EAE) in C57BL/6 mice. 63 female mice (6–8 weeks) chosen and randomly divided into three groups: control, low-dose rapamycin-treated EAE (0.3 mg/kg), high-dose (1 mg/kg). The recovery neurological function concentrations were assessed by score; assessment periods: initial stage (10-13d), peak phase (17-21d), remission (25-28d), calculated score for each period. inflammatory cell infiltration IL-17 A immunohistochemical staining which produced Th17 positive count. evaluated flow cytometry expression CD4+ CD25+ Foxp3+ T cells. transcription factors RORC (RAR-related orphan receptor C) mRNA qRT-PCR Treg cells In score, group significantly lower than other two groups drug (P 0.05). percentage CD4+CD25+Foxp3+T cells, number Foxp3 level greater those vehicle-treated group. High (1 mg/kg) have a better relieves inflammation altering balance Treg/Th17 mouse model.
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