Ultrasensitive and accelerated detection of ciguatoxin by capillary electrophoresis via on-line sandwich immunoassay with rotating magnetic field and nanoparticles signal enhancement.
作者： Zhaoxiang Zhang , Chaoying Zhang , Wenxiu Luan , Xiufeng Li , Ying Liu
DOI: 10.1016/J.ACA.2015.06.018
关键词:
摘要: A sensitive and rapid on-line immunoassay for the determination of ciguatoxin CTX3C was developed based on a capillary mixing system, which integrated with electrophoresis (CE) separation electrochemical (EC) detection. In sandwich anti-CTX3C-functionalized magnetic nanoparticles were used as immunosensing probes, horseradish peroxidase (HRP) anti-CTX3C antibody bound onto surface gold (AuNPs) recognition elements. Online formation immunocomplex realized in inlet end an external rotating field. Compared classical HPLC-MS ELISA, assay adopting AuNPs multienzyme carriers online format field exhibited higher sensitivity shorter time. The linear range from 0.6 to 150 ng/L correlation coefficient 0.9948 (n = 2), detection limit (S/N 3) 0.09 ng/L. showed satisfying reproducibility stability, it successfully applied quantification fish samples.
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Shaorong Liu, Purnendu K. Dasgupta, Electroosmotically pumped capillary flow-injection analysis. Valve-based injection systems and sample throughput Analytica Chimica Acta. ,vol. 283, pp. 739- 745 ,(1993) , 10.1016/0003-2670(93)85288-U
Fei Liu, Yongming Li, Chaojun Song, Bangquan Dong, Zhijia Liu, Kui Zhang, Haitao Li, Yuanjie Sun, Yuying Wei, Angang Yang, Kun Yang, Boquan Jin, None, Highly sensitive microplate chemiluminescence enzyme immunoassay for the determination of staphylococcal enterotoxin B based on a pair of specific monoclonal antibodies and its application to various matrices. Analytical Chemistry. ,vol. 82, pp. 7758- 7765 ,(2010) , 10.1021/AC101666Y
Xiao-Wei Zhang, Zhao-Xiang Zhang, Quantification of domoic acid in shellfish samples by capillary electrophoresis-based enzyme immunoassay with electrochemical detection. Toxicon. ,vol. 59, pp. 626- 632 ,(2012) , 10.1016/J.TOXICON.2012.02.011
Adriano Ambrosi, Federico Airò, Arben Merkoçi, Enhanced gold nanoparticle based ELISA for a breast cancer biomarker. Analytical Chemistry. ,vol. 82, pp. 1151- 1156 ,(2010) , 10.1021/AC902492C
Andrea Ranzoni, Gwenola Sabatte, Leo J. van IJzendoorn, Menno W. J. Prins, One-step homogeneous magnetic nanoparticle immunoassay for biomarker detection directly in blood plasma. ACS Nano. ,vol. 6, pp. 3134- 3141 ,(2012) , 10.1021/NN204913F
Ying Liu, Quan Cheng, Detection of Membrane-Binding Proteins by Surface Plasmon Resonance with an All-Aqueous Amplification Scheme Analytical Chemistry. ,vol. 84, pp. 3179- 3186 ,(2012) , 10.1021/AC203142N
Richard J. Lewis, Michelle Sellin, Recovery of ciguatoxin from fish flesh Toxicon. ,vol. 31, pp. 1333- 1336 ,(1993) , 10.1016/0041-0101(93)90407-A
Ali Akbar Asgharinezhad, Homeira Ebrahimzadeh, Fatemeh Mirbabaei, Narges Mollazadeh, Nafiseh Shekari, Dispersive micro-solid-phase extraction of benzodiazepines from biological fluids based on polyaniline/magnetic nanoparticles composite. Analytica Chimica Acta. ,vol. 844, pp. 80- 89 ,(2014) , 10.1016/J.ACA.2014.06.007
Dianping Tang, Biling Su, Juan Tang, Jingjing Ren, Guonan Chen, Nanoparticle-based sandwich electrochemical immunoassay for carbohydrate antigen 125 with signal enhancement using enzyme-coated nanometer-sized enzyme-doped silica beads. Analytical Chemistry. ,vol. 82, pp. 1527- 1534 ,(2010) , 10.1021/AC902768F
Xiaoli Wang, Yuling Song, Maoyong Song, Zhixin Wang, Tao Li, Hailin Wang, Fluorescence polarization combined capillary electrophoresis immunoassay for the sensitive detection of genomic DNA methylation. Analytical Chemistry. ,vol. 81, pp. 7885- 7891 ,(2009) , 10.1021/AC901681K