Influence of high-fat diet on bone tissue: An experimental study in growing rats
作者： G. Rezende Yanagihara , R. Carminati Shimano , J. Atsuko Tida , J. Suzuki Yamanaka , S. Yasuyo Fukada
DOI: 10.1007/S12603-017-0871-X
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摘要: The relationship between obesity and bone tissue remains contradictory, especially when the effect of high-fat diet is assessed in experimental models. aim this study was to evaluate effects on metabolism growing rats. Twenty weaned female Wistar rats were equally divided into two groups: SD (standard diet) HFD (high-fat with 60 % energy as fat). After five weeks diets, euthanized, liver, blood bones extracted. liver analysed for malondialdehyde (MDA) reduced glutathione (GSH) concentrations. Blood by ELISA method osteoprotegerin (OPG) tumour necrosis factor ligand superfamily member 11 (TNFSF11/RANKL). dual-energy X-ray absorptiometry (DXA), mechanical tests, computed microtomography, histological quantitative analysis scanning electron microscopy. gene expressions PPAR-γ Runx-2, RANKL Cathepsin-K also evaluated. caused an increase MDA concentration, indicating oxidative stress. It increased expression PPAR-γ, which that related adipocyte differentiation. There BMD tibia animals fed HFD, but other microstructural properties maintained unaltered. In addition, there no changes differentiation osteoblasts osteoclasts, well biochemical markers formation resorption. Liver parameters are changed response HFD. However, although BMD, microstructure function did not change after a 5-week
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