摘要: Abstract 1. The kinetics of the system: ascorbate-cytochrome c-cytochrome oxidase-oxygen, have been studied, using Keilin and Hartree horse heart-muscle preparation. 2. In disagreement with general belief, exogenous cytochrome c is not “nearly completely” reduced under conditions these measurements. 3. reduction ferricytochrome by ascorbate first order respect both to ascorbate. 4. No explanation for observed could be found on basis that only function reduce c, oxidation latter following same as in absence 5. could, however, explained if reduces but enters enzymic reaction a second substrate. significance this possible path discussed. 6. effects temperature inhibition azide studied.
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sci-hub.se 参考文章(24)
Lucile Smith, Reactions of cytochromes a and a3. I. Studies of oxidation and reduction of the pigments in a purified preparation. Journal of Biological Chemistry. ,vol. 215, pp. 833- 846 ,(1955) , 10.1016/S0021-9258(18)66006-5
K. W. Cleland, E. C. Slater, Respiratory granules of heart muscle Biochemical Journal. ,vol. 53, pp. 547- 556 ,(1953) , 10.1042/BJ0530547
Andrew Hunter, Clarence E. Downs, THE INHIBITION OF ARGINASE BY AMINO ACIDS Journal of Biological Chemistry. ,vol. 157, pp. 427- 446 ,(1945) , 10.1016/S0021-9258(18)51079-6
Takashi Yonetani, Studies on cytochrome oxidase. II. Steady state properties. Journal of Biological Chemistry. ,vol. 235, pp. 3138- 3143 ,(1960) , 10.1016/S0021-9258(20)81325-8
Elmer Stotz, Aaron M. Altschul, T.R. Hogness, THE CYTOCHROME C-CYTOCHROME OXIDASE COMPLEX Journal of Biological Chemistry. ,vol. 124, pp. 745- 754 ,(1938) , 10.1016/S0021-9258(18)74035-0
E. C. Slater, The measurement of the cytochrome oxidase activity of enzyme preparations Biochemical Journal. ,vol. 44, pp. 305- 318 ,(1949) , 10.1042/BJ0440305
Philip George, The Specific Reactions of Iron in Some Hemoproteins Advances in Catalysis. ,vol. 4, pp. 367- 428 ,(1952) , 10.1016/S0360-0564(08)60619-7
John Beetlestone, The oxidation of cytochrome c by cytochrome c peroxidase. Archives of Biochemistry and Biophysics. ,vol. 89, pp. 35- 40 ,(1960) , 10.1016/0003-9861(60)90008-4
Philip George, G. I. H. Hanania, The haem-linked ionizing group in myoglobin Discussions of the Faraday Society. ,vol. 20, pp. 216- 224 ,(1955) , 10.1039/DF9552000216
Milton Kern, Efraim Racker, Activation of a DPNH oxidase by an oxidation product of ascorbic acid. Archives of Biochemistry and Biophysics. ,vol. 48, pp. 235- 236 ,(1954) , 10.1016/0003-9861(54)90329-X