A novel immobilization fluorescence capillary analysis method and its applications

摘要: Fluorescence capillary analysis (FCA) realizes trace-level of micro-volume samples; it is easy to operate, extremely low in analytical cost and can significantly lessen environmental pollution from chemistry waste. FCA has the characteristics green been applied clinical, biochemical, pharmaceutical, food safety other fields. basically involves a glass capillary, holder an ordinary fluorescence detector. The not only container for chemical reaction detection but also functions as carrier immobilize enzymes, gene probes or reagents; be used repeatedly disposable. In analysis, which modified with functional reagents sucks measured liquid then inserted into within fluorescent detector measurement. immobilized method successfully determination reduced coenzyme I, ethanol liqueur, lactic acid dairy products, pyruvic glucose serum, sulfated bile urine, ratio pyruvic/lactic cells well DNA end-labeling dyeing methods. Further, extended arrays complete multipurpose simultaneous determinations combined mobile phones detectors use health technology. will produce considerable social benefits medicine, pharmacy, fermentation food, protection Therefore, relevant contents are presented this tutorial review.