Immunomodulation of murine experimental autoimmune encephalomyelitis by pertussis toxin: the protective activity, but not the disease-enhancing activity, can be attributed to the nontoxic B-oligomer.

摘要: Bordetella pertussis and its major virulence component, toxin (PT), have been used routinely to promote the development of such murine experimental autoimmune diseases as encephalomyelitis (EAE). Recently, we reported that B. also can protect against EAE. The protective activity was assigned PT, a complex holomer composed an A-promoter, toxic S1 subunit, B-oligomer comprised subunits S2, S3, S4, S5. Although some data are available explain how PT enhance EAE, nothing is known about mechanism by which it protects disease. Toward understanding conflicting effects on investigated immunomodulatory various components PT. Herein show enhancing activities reside within different regions holomer. Thus, though appeared essential in imparting played no role importance disease protection, could be fully B-oligomer. Further investigation with gel-purified revealed protected EAE varying extent, S3 being most protective. These suggest potential therapeutic application for or subunits, appear potent agents, without toxicity disease-promoting associated