Development of an in vitro macrophage system to assess Penicillium marneffei growth and susceptibility to nitric oxide.

摘要: We investigated the effect of nitric oxide (NO) and reactive nitrogen intermediates on in vitro growth Penicillium marneffei both a cell-free system novel macrophage culture system. In system, NO that was chemically generated from NaNO2 acid media (pH 4 5) markedly inhibited P. marneffei. On contrary, inhibition did not occur neutral medium 7.4) which produced. conidia were phagocytized by nonstimulated murine J774 macrophages after 2 h incubation. During following 24 h, grew as yeast-like cells replicating fission macrophages. The intracellular damaged macrophages, confirmed electron microscopy. When stimulated gamma interferon lipopolysaccharide, led to enhanced production intermediates, percentage significantly reduced synthesis N-monomethyl-L-arginine restored inverse correlation between intramacrophage amount nitrite detected supernatants supports hypothesis L-arginine-dependent pathway plays an important role immune response against