Overall biochemical changes in bacteria photosensitized with cationic porphyrins monitored by infrared spectroscopy

作者: Eliana Alves , Catarina Moreirinha , Maria AF Faustino , Ângela Cunha , Ivonne Delgadillo

DOI: 10.4155/FMC-2015-0008

关键词:

摘要: Background. Photodynamic inactivation of micro-organisms is a promising nonantibiotic multitarget approach to treat localized and superficial infections through oxidative stress. Herein, the changes occurring on major cellular components Escherichia coli Staphylococcus warneri, induced by photosensitization with cationic porphyrins (Tri-Py+-Me-PF Tetra-Py+-Me) white light, were monitored infrared spectroscopy. Results: In E. coli, most occurred proteins lipids, suggesting key effect lipopolysaccharides in first irradiation times. S. molecular targets damage but phospholipids polysaccharides also affected. Conclusion: Infrared spectroscopy very interesting tool monitor biochemical bacteria infer its mechanism action.

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